Thioglycollate Broth

Thioglycollate Medium is used for the sterility testing of certain biological products which are turbid or viscous. The medium is highly enriched and supports the growth of pathogenic bacteria. Tryptone serves as a source of nitrogen and carbon compounds, long chain amino acids and other essential nutrients. Yeast extract serve as source of essential nutrients to the contaminants, if present. Dextrose serves as the energy source. Sodium chloride maintains the osmotic equilibrium of the medium whereas L-cystine, an amino acid, also serves as source of essential growth factors.